ImmunoChemistry Technologies

268 products

ImmunoChemistry Technologies is your partner in cell viability assays, ELISA reagents, IHC reagents, and immunoassay services. With decades of protein chemistry expertise, ICT offers a wide range of products for immunoassay, cellular, and molecular diagnostics development. Our kits include fluorescent whole cell assays for intracellular apoptosis detection, autophagy, oxidative stress, caspase activity, and more. Optimize your technique with our coating and wash buffers, blockers, sample and assay diluents, stabilizers, and stop solutions. From ELISA development and plate coating, to IHC counterstains and mounting media, to antibody conjugation and purification, the team at our ISO-13485-certified facility in Davis, California is dedicated to helping you build a better assay.

Showing 1 - 24 of 268 products

Figure 1. THP-1 cells were treated with either a negative control (A), or PMA (5 ng/mL) followed by LPS (10 ng/mL). Cells were then stained with FAM-YVAD-FMK (cat. 97/98) and analyzed by phase contrast and fluorescence microscopy. In the treated sample, many cells appear bright green, indicating increased caspase-1 activity (B). In the non-induced sample, few green cells are visible, indicating a low level of caspase-1 activity (A). Data courtesy of Dr. Brian Lee, ICT (207:11).

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-1 (YVAD) Assay Kit

From $234

Figure 1. Flow cytometry analysis of active caspase-9 in MDA-MB-231 (A,B) and ZR-75-1 (C,D) cells. Cells were stained with FAM-FLICA® Caspase-9 reagent. From Figure 5 of R. Krętowski and M. Cechowska-Pasko, The Reduced Graphene Oxide (rGO) Induces Apoptosis, Autophagy and Cell Cycle Arrest in Breast Cancer Cells, Int J Mol Sci (2022) 23, 9285. Used under the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). Copyright © 2022 by the authors.

ImmunoChemistry Technologies Green Fluorescent FAM-FLICA® Caspase-9 (LEHD) Assay Kit

From $216

ImmunoChemistry Technologies ELISA Wash Buffer, 10X

From $44.75

Figure 1. Signals leading to activation of the NLRP3 inflammasome.

Figure 2. C2BBe1 cells were infected with wild type Salmonella constitutively expressing mCherry (red). After 9 hours, live cells were incubated with FAM-YVAD-FMK (green cell) for 1 hour. The infected cell (red) is undergoing pyroptosis, and shows increased green fluorescence compared to uninfected cells due to caspase-1 activation. Data courtesy Knodler, Leigh A., et al. Dissemination of invasive Salmonella via bacterial-induced extrusion of mucosal epithelia. PNAS. 107:41, 17733-17738 (2010).

ImmunoChemistry Technologies Pyroptosis/Caspase-1 Assay - Green Fluorescent

From $340.50

Figure 1. Cresyl Violet Perchlorate Excitation and Emission Spectra in Ethanol

Figure 2. Intracellular cathepsin activity was detected in HL60 cells using Magic Red®-(LR)2 cathepsin K fluorogenic substrate. Intracellular localization of the hydrolyzed fluorescent Magic Red® product was detected using a Nikon Eclipse E800 photomicroscope equipped with a 510–560 nm excitation filter and a 570–620 nm emission/barrier filter at 500X (A). Photo at right (B) shows the corresponding DIC image of the cells. Data courtesy of Dr. Brian Lee, ICT, 061202.

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin K Assay Kit

From $206

Figure 2. MCF-7 cells were stained with acridine orange for 30 minutes, then washed twice in PBS (cells not stained with Magic Red®) and then analyzed by fluorescence microscopy at 400X using either 492 nm excitation with a 540-550 nm emission filter (A, lysosomes appear yellowish green), or 540 nm excitation with a long pass >640 nm barrier filter (B, lysosomes appear red). Data from the laboratory of Dr. Z. Darzynkiewicz (Brander Cancer Research Center Institute, New York City, NY).

ImmunoChemistry Technologies Magic Red® Fluorescent Cathepsin B Assay Kit

From $206

ImmunoChemistry Technologies Tetramethylbenizidine IHC Peroxidase

From $85.75

ImmunoChemistry Technologies Alkaline Phosphatase Enhancer

$113.75

ImmunoChemistry Technologies BCIP/NBT

From $68.25

ImmunoChemistry Technologies Fast Red Super ™

$113.75

ImmunoChemistry Technologies Peroxidase Signal Enhancer Kit

$113.75

ImmunoChemistry Technologies AEC Peroxidase Chromogen Substrate Kit

$113.75

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Rabbit Serum

From $58

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Horse Serum

From $58

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Goat Serum

From $58

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Donkey Serum

From $58

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Chicken Serum

From $58

ImmunoChemistry Technologies Immuno Protein Blocking Solution with Bovine Serum

From $58

ImmunoChemistry Technologies Levamisole, Alkaline Phosphatase Endogenous Blocking Reagent, 15X

From $68.25

ImmunoChemistry Technologies Endogenous Peroxidase Block

From $58

ImmunoChemistry Technologies Avidin/Biotin Blocking Kit Set

From $74.50

ImmunoChemistry Technologies Dual (Peroxidase & Alk Phos) Endogenous Blocking Reagent

From $58

ImmunoChemistry Technologies Streptavidin/Biotin Blocking Kit Set

From $74.50

ImmunoChemistry Technologies BSA (IgG and Protease free)

From $68.25

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